S4108 Tissue Engineering Practical Course

Kursbeschreibung / -kommentar

The primary aim of this course is to become familiar with the basics of cell and tissue culture. The students will be introduced to basic laboratory techniques doing actual bench work. It is intended for biomedical engineers that are “beginners” of cell culture and tissue engineering. A strong weight will be given to 3D culture systems, such as hydrogels but also to the most commonly used protocols to characterise a culture system, such as proliferation, activity, and matrix production, e.g. DNA content, glycosaminoglycan content, cell viability and cell activity, respectively. Students will learn how to maintain cells in culture in 2D but also in 3D culture using hydrogels. Furthermore, students have the opportunity to familiarise with proper pipetting and handling of liquids. These skills will be exercised within the framework of a 2-week experiment where primary cells will be seeded in 3D carriers, i.e. alginate, agarose and fibrin. These culture systems will be compared to the 2D monolayer. The theory and own results of the practical exercises are discussed between the experimental work.
The course is completed with a scientific report at the end of the course.

Requires previous module: Course WN403 Tissue Engineering I

In this course important aspects of sterile cell culture of mammalian cell culture shall be trained. Proper handling of micropipettes, pipette boys, liquids shall be trained. The course provides an overview for basic methods and assays, which are used in tissue engineering.